胶原蛋白水解物的生物利用度和对犬骨关节炎的影响（从体内到体外）.pdf

1、Niels Blees(N.R.),DVM,MScFaculty of Veterinary Medicine,Utrecht UniversityBioavailability and effects of collagen hydrolysates in canine osteoarthritisOsteoarthritisOsteoarthritisOsteoarthritisAn incurable diseaseHealthy jointOsteoarthritisMade with BiorenderMultimodal supportCollagen hydrolysates h

2、ave potential for joint supportBlees et al.,2024Assessing bioavailabilityBlees et al.,2024Diet x 750 x 250 x 500250 x 750 x Diet x 500750 x 250 x 500 x Diet x x xAssessing bioavailabilityAssessing Bioavailability060060060060050100Free hydroxyprolineTimepoint(min)Free Hyp Concentration(M)p 0.001p 0.0

3、01p=0.003p 0.001p 0.0010600600600600100200300Total hydroxyprolineTimepoint(min)Total Hyp Concentration(M)0 mg/kg250 mg/kg500 mg/kg750 mg/kgp 0.001p 0.001p 0.001p=0.045p=0.005Assessing Bioavailability060060060060050100Free hydroxyprolineTimepoint(min)Free Hyp Concentration(M)p 0.001p 0.001p=0.003p 0.

4、001p 0.0010600600600600100200300Total hydroxyprolineTimepoint(min)Total Hyp Concentration(M)0 mg/kg250 mg/kg500 mg/kg750 mg/kgp 0.001p 0.001p 0.001p=0.045p=0.005All doses higher than control at T60No cumulative effect on baselineNo difference between 500-750 mg/kgFrom in vivo to in vitro0 mg/kg 250

5、mg/kg 500 mg/kg 750 mg/kgFrom in vivo to in vitro-Synovial cellsSynovial CellsSynovial CellsSynovial CellsMild anticatabolic effectsHeterogeneous,donor-dependent responseFrom in vivo to in vitroCartilage-from RNA to proteinAnti-fibrotic?Cartilage-from RNA to proteinAnti-fibrotic?Again:Heterogeneous inter-donor responseConclusions?CH is well-absorbed in dogsPotential anti-catabolic andanti-fibrotic effectsConclusionsThank you!Utrecht UniversityMichelle TeunissenRonald CorbeeMarianna TryfonidouSonacCarine van VurenEsther Prevo-Keijbets