PFAS 风险评估和毒理学研究.pdf

1、Kurt GustEnvironmental Laboratory(EL)USACE Engineer Research&Development CenterE-mail:kurt.a.gustusace.army.milPhone:601-634-3593PFOS Bioaccumulation and Bioconcentration Factors in Multi-Generational Zebrafish ExposuresDisclaimer StatementThe contents of this presentation neither constitute nor nec

2、essarily reflect DOD policy.AcknowledgementsResearch Team:Ashley N.KimbleJ.Erik MylroieMichael L.MayoMitch S.WilbanksCatherine S.C.StewardKacy A.ChapmanGuilherme R.LotufoNatalia Garcia-ReyeroDavid W.MooreSponsors:SERDP ESTCP Sediment Management Work Group(SMWG)*Select membersBackgroundPer-and polyfl

3、uoroalkyl substances(PFAS)pose exposure risks to diverse ecological systems and to humans.The PFAS,perfluorooctane sulfonic acid(PFOS),is broadly distributed in surface waters and is toxic to a variety of aquatic organisms including fish.A need exists to understand how PFOS bioaccumulates in aquatic

4、 exposures.In the Present Study We determined how exposure concentrations,exposure durations,and developmental life stages affected PFOS accumulation in zebrafish.Maternal transfer of PFOS to eggs was quantified.A toxicokinetic model was developed that accurately predicts PFOS body burdens in multip

5、le zebrafish life stages spanning broad exposure durations.Multi-Generational Exposure(Conceptual Overview)Survival Measured DailyGrowth Measured at 30d,60d,90d,and 180d.Reproduction Beginning at 4 months and measured 8 times for both P and F1.Tissue Residue Analysis P Generation at 14d,29d,and 180d

6、 and F1 Generation at 180d.RNA seq,Vitellogenin&Histopath.End of each generation.F2(16d Exposure)Gust et al.2024 Environ Toxicol Chem 43(1):115-131,DOI:10.1002/etc.5770Experimental Design(Continuous Multi-Gen Exposure)Design Details:5 PFAS Concentration+Control5 Replicate Tanks/Treatment50 Fish per