精准发酵与细胞培养.pdf

1、Lecture#5Precision Fermentation and Cell-Cultivated ProteinsTodays agenda:Precision Fermentation DetailsCell-Cultivated Protein LandscapeCell-Cultivated Cultures&ProcessSustainable Food Proteins CourseMinneapolis October,2025Simplified Precision Fermentation Process Flow Engineered microorganisms to

2、 produce specific functional ingredients in a controlled and efficient mannerEng.Strain(Yeast or Fungi)Feed-StockCentrifuge FiltrationWet BiomassDried BiomassPrecision MaterialLiquid FractionPurificationConcentrationDryingExamples of companies within the PF Landscape:Most PFMany enabling technology

3、companies assistingFermentation Types and DetailsBatchFed-BatchContinuousPerfusionEfficiency+Complexity and Novelty2-5%10-20%2-10%20-30%Cell DensityCell DensityCell DensityCell DensityDefinition:Definition:Definition:Definition:Feedstock&strain added in fermentation tank inoculate cells,growth,then

4、all of the tank is harvestedGas release monitored to determine when additional feedstock should be added.Allows for increased cell density if gas can be stirred in.Feedstock added throughout process AND waste build-up is removed at a certain point.This allows for increased cell density compared to F

5、ed-Batch.Feedstock added constantly&waste build-up constantly removed,creating a steady state of inputs and outputs.Used primarily for slow growing cells.All Cell-Cultivatedg/L maxg/L maxg/L maxg/L max20-50100-20020-100200-300g/L/h0.5-2g/L/h2-5g/L/h1-3g/L/h3-10Total:1-7 daysYongky A,Xu J,Tian J,Oliv

6、eira C,Zhao J,McFarland K,Borys MC,Li ZJ.Process intensification in fed-batch production bioreactors using non-perfusion seed cultures.MAbs.2019 Nov-Dec;11(8):1502-1514.doi:10.1080/19420862.2019.1652075.Epub 2019 Aug 19.PMID:31379298;PMCID:PMC6816350.Jianfeng Xu,Xumeng Ge,Maureen C.Dolan,Towards hig