田克恭-PCV3、PEDV基因工程亚单位疫苗的创制.pdf

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1、PCV3、PEDV基基因因工工程程亚亚单单位位疫疫苗苗的的创创制制Development of Genetic Engineering Subunit Vaccine of PCV3 and PEDV普莱柯生物工程股份有限公司国家兽用药品工程技术研究中心Pulike Biological Engineering Co.,Ltd.National Engineering Research Center for Veterinary Drugs田田克克恭恭Tian Kegong2024.04.26 郑州April 26,2024 Zhengzhou一带一路国际生猪产业大会IPCV3亚单位疫苗的创制

2、Development of PCV3 Subunit Vaccine二IIPEDV亚单位疫苗的创制Development of PEDV Subunit Vaccine目录Table of Contents一带一路国际生猪产业大会PCV3亚亚单单位位疫疫苗苗的的创创制制Development of PCV3 Subunit VaccineI一带一路国际生猪产业大会主主要要内内容容Main contents2.PCV3 VLPs的的表表达达2.Expression of PCV3 VLPs3.PCV3 VLPs工工艺艺开开发发3.PCV3 VLPs Process Development1.P

3、CV3概概述述1.Overview of PCV3PCV3病病原原学学分分析析PCV3 Etiological AnalysisPCV的的发发现现史史History of PCVPCV3流流行行现现状状Epidemiological status of PCV3Cap序序列列分分析析、优优化化Cap sequence analysis and optimization表表达达载载体体改改造造Expression vector modification表表达达细细胞胞构构建建Expression Cell ConstructionPCV3 VLPs表表征征PCV3 VLPs Characteri

4、zationPCV3 VLPs表表达达工工艺艺开开发发PCV3 VLPs Expression Process DevelopmentPCV3 VLPs纯纯化化工工艺艺开开发发PCV3 VLPs Purification Process Development5.PCV3 VLPs疫疫苗苗创创制制5.PCV3 VLPs vaccine development疫疫苗苗安安全全性性Vaccine safety疫疫苗苗效效力力Vaccine efficacy4.PCV3诊诊断断试试剂剂开开发发4.Development of PCV3 diagnostic reagent特特异异性性单单抗抗制制备备

5、Preparation of specific monoclonal antibodies阻阻断断ELISA的的开开发发Development of Blocking ELISA阻阻断断ELISA的的评评价价Evaluation of Blocking ELISA阻阻断断ELISA的的临临床床应应用用Clinical application of blocking ELISA一带一路国际生猪产业大会病病原原：PCV3属于圆环病毒科、圆环病毒属成员；通过呼呼吸吸道道和消消化化道道传播。Pathogen:PCV3 belongs to the Circoviridae family and the

6、 Circovirus genus;spread through respiratory tractand Digestive tract.临临床床表表现现和和危危害害：母猪体表多处出现丘疹性皮炎，可造成怀孕母猪流产、死胎和木乃伊胎等；断断奶奶仔仔猪猪表现为食食欲欲下下降降、发发热热和和先先天天性性震震颤颤等；所所有有年年龄龄段段仔猪均可感染，小鼠、犬、牛亦可感染。Clinical manifestations and hazards:papular dermatitis appears on many parts of the sows body surface,which can caus