1、Gene SynthesisHandbookFourth EditionA Guide to Gene Synthesis Design and ApplicationsAdditional FeaturesNEVER MISSA DEADLINE AGAINENSURE YOUR DNAQUALITYALWAYS HAVEA BACKUP PLANGenSmartTM Fast Ordering System GenScript Safeguard SystemOur 9-step process ensures DNA fidelity&purityISO 9001-Certified Q
2、uality ManagementPrecision and accuracy with every orderCloneArkTM Plasmid Storage System Plasmid recovery in the event of an accidentAutomated,High-Throughput PlatformFrom design to order in less than 3 minutes!Orders of any size with the fastest delivery timesAs fast as 4 days!Custom Gene Synthesi
3、sCustom Gene SynthesisTake Control of Your Research with GenS PhD-level Support at Every StepGene Expertise fromOver 600,000 ProjectsIndustry-leading OptimumGeneCodon Optimization TechnologyPlasmidfidelityguaranteedTable of ContentsApplicationsTechnologyDefinition and history Types of synthetic prod
4、ucts SignificanceSequence design and applicationsSynthesisAssemblySequence verification and error correctionDownstream application preparation35734374045461313152017Perspectives BiosafetyFuture5252Resources ToolsDesign considerations5556Core applications Broad applications Generating nucleic acid-ba
5、sed reagentsCodon optimizationMutagenesis 202831Synthetic biology Translational research and medicineAgriculture and environment Introduction1IntroductionChapter OneDefinition and HistoryGene synthesis refers to the base-by-base process of chemically synthesizing a strand of DNA.Unlike traditional m
6、ethods of DNA replication,gene synthesis does not require a template strand.Rather,gene synthesis involves the step-wise addition of nucleotides to a single-stranded nucleic acid molecule,which then serves as a template for the synthesis of the complementary strand.Sequences with varying lengths can