追踪食品中的沙门氏菌和李斯特菌：快速检测和菌株鉴别.pdf

1、Rapid DetectionChasing Salmonella and Listeria in Food:Rapid detection and strain discriminationOlaf Degen,Director Industry MicrobioogyBruker 09 May 2024|2023 BrukerBRUKER MICROBIOLOGY&INFECTION DIAGNOSTICS2 2SalmonellaCronobacterColiformsCampylobacterProteomics Technology:MALDI-TOF MS-you can util

2、ize it forFood&Industrial Microbiology13/05/2024L.monocytogenesLactic acid bacteriaYeasts&MoldsFilamentous Fungiconfirmation of:identification of:Degen,Food Safety Summit 2024|2023 BrukerXLD AgarGRAM-NEGATIVE FOOD PATHOGENSDAY 1DAY 1Liquid enrichmentDAY 2DAY 2Transfer to selectiveagars:Streak on XLD

3、&Chromogenic Agar-Incubation:37.0C,24hDAY 3DAY 3Presumptive Salmonella,needs confirmationSAVE workload and Time-to-resultISO ISO 6579:Salmonella Salmonella ScreeningISO-confirmationConfirmation byMALDI BiotyperDAY 4&5DAY 4&5(normal workflow):Confirmation includes plating on non-selective Agar plates

4、 for 24h and biochemical tests.In many cases followed by Salmonella serology(subtyping).3 3Quality control of ingredientsEnd product checkA typical customer application:Confirmation of SalmonellaSamplingEnvironmental monitoringFood&Industrial Microbiology13/05/2024Degen,Food Safety Summit 2024|2023

5、BrukerMALDI-TOF MS FOR FOOD MICROBIOLOGYMALDI BiotyperprincipleSalmonellaListeriaIdentify Gram+,Gram-bacteria,yeasts and moldsin minutes,by their unique proteomic fingerprint Non-selectiveor selectiveagars4 413.05.2024Innovation with Integrity Food and Industrial MicrobiologyCronobacter|2023 Bruker|

6、2023 BrukerMALDI Biotyper ISO 16140 validated,plusAOAC-OMA method and method of USDA-FSIS laboratory guidebookMALDI BiotyperMALDI Biotyperhas been successfully tested by the has been successfully tested by the USDAUSDA-Food Safety and Inspection Service(FSIS).Food Safety and Inspection Service(FSIS)