猪轮状病毒感染 – 生猪产业中不容忽视的重要问题及解决方案冯力哈尔滨兽医研究所.pdf

1、Porcine rotavirus(PoRV)infection and control strategy：an important problem in pig industry 猪轮状病毒感染：生猪产业中不容忽视的重要问题及解决方案 Epidemic investigation of PoRVPathogenicity of PoRVContentsStrategies against PoRV infectionBackgroud-classificationReoviridaeRotavirusdsRNA virusVP1VP4、VP6、VP7;NSP1NSP5Discovered a

2、nd isolated in 1973.One of the important enteric pathogens in children and in a wide range of young ainimals.ABCDEFGHIJBased on VP6ACHBackgroud-characteristicsMorbidity ：day 1-7 piglet 80%Mortality ：7%20%Symptom:Diahhrea and EmesisHigh resistance to temperature,humidity and pHNo loss for infectivity

3、 at 4,drynessNo significant change for infection with the season changeDistribution differences of RVA strains in different regions（Hajnalka Pappet al.，2013）Backgroud-main genotypes in pigsQ1:Now main genotypes in pig industry?Q2:The pathogenicity is high or low?Q3:How to solve RVs infection?Part 1-

4、Epidemic investigation of PoRV1.1 Establishing of VP7 and VP4 gene PCR method and analysis of its genetypeVP4VP753M2000bp1000bpFig.1 Identification of VP7 by PCR图1 PCR扩增VP7基因InformationLocation for sampling：黑龙江、河北、内蒙、甘肃、山东、江苏、湖南、四川、广东Target：RVA、RVCGene tested：VP7 and VP8*Time：2020-20211.2Epidemic in

5、vestigation of PoRV1.2 PoRV A VP7Tips：1.G9,G5,G12,G4 and G3 are the main genotype；2.Mixed infection with at least two genotypes；1.3 PoRV A VP4Tips：1.P23,P13 and P7 are the mian genotype；1.4 PoRV C VP7 and VP4VirusNumberLocationG6P56成都成都南京南京G1P51南京南京G9PX1成都成都1.5 Isolation and passageG1P7、G12P7、G9P23、

6、G5P23Passage and adaptation 108 TCID50/ml Identification of VP7 by PCR1.6 Cross neutralization among diffetent strainsG5P23G5P23G9P23G9P23G1P7G1P7G12P7G12P7G1P74008003200400G12P740040016006400G5P231280032001600200G9P2312800512003200400Hyperimmune SerumViruses（103 TCID50）Note：Negative serum100Part 2-