开创高价值鳍鱼的未来：养殖海鲜产品开发.pdf

1、 2025 BLUENALU,INC.,PROPRIETARY&CONFIDENTIAL Pioneering the future of high-value finfish:Cultivated seafood product developmentCourtney Benson,Ph.D.Director,R&DMy background B.S/M.S in Cell&Molecular Biology and Ph.D.in Neuroscience President of the Association for Women in Science(AWIS)From microbe

2、s to neurodevelopment and schizophrenia,my research led me to cell line development and ultimately to BlueNalu.Key accomplishments at BlueNalu:Developing finfish cell linesDeveloping lipid loading in finfish3 2025 BLUENALU,INC.,PROPRIETARY&CONFIDENTIAL BlueNalu is a next generation seafood company d

3、elivering renewable protein,without taking fish from the ocean.hIGH-VALUE finfish Prototypes Yellowtail amberjack fall 2019Mahi mahi summer 2021Bluefin tuna summer 2022Scalable process from cell to sushiCell line developmentFlexible cell line platform technologyBlueNaludeveloped a platform technolog

4、y to routinely generate cells lines from a wide range of fish speciesSpeciesSelection StrategyoVulnerable in the wildoDifficult to farm-raiseoHigh in contaminantsoPrimarily importedoDrives premium price pointCell types of interestinclude muscle,fat,&connective tissue Non-GMO cells Scalable cell-line

5、 Cells in suspension Animal-Component Free MediaCLD GuidepostsCELL LINE DEVELOPMENT OVERVIEW Stable line generation(2D)Stable line generation(2D)Primary cells are stabilized in adherent culture to achieve reproducibility and longevityDifferentiation and lipid loadingDifferentiation and lipid loading

6、 Identify lines with high differentiation to muscle or fat Lipid loading enables decreased cost of production Suspension cell line generationSuspension cell line generation Adaptation from 2D to suspension enables easier scale-up of cell cultures Faster growth rate improves commercial production vol